Coding

Part:BBa_K2736105:Design

Designed by: Lorenzo Pattiradjawane   Group: iGEM18_Utrecht   (2018-10-10)


Tar methylation mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

Source

This part was created by site directed mutagenesis of BBa_K777001 with a forward primer containing the sequence 5'GCATCGCTGGTGCAGGCGTCAGCTGCCGCCGCC, and a reverse primer containing the sequence 5'GGCGGCGGCAGCTGACGCCTGCACCAGCGATGC.

References